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December
14, 2002
| Procyanidin B-3,
isolated from barley and identified as a hair-growth stimulant, has the
potential to counteract inhibitory regulation by TGF-1 |
| Ayako Kamimura and Tomoya
Takahashi Abstract: With the aim of
identifying natural products, which possess hair-growing activity, we
examined more than 1000 plant extracts with respect to their
growth-promoting effects on hair epithelial cells. We discovered intensive
growth-promoting activity, about 140% relative to controls, in barley
extract. Our strategy for identifying active compounds in barley extract
involved subjecting it to column chromatography using HP-20 resin columns,
an LH-20 resin column, and preparative high-performance liquid
chromatography (HPLC) using an ODS column. The 60% (v/v) aqueous methanol
eluted fraction from the HP-20 column and the 75% (v/v) aqueous methanol
eluted fraction from the subsequent LH-20 column showed high hair-growing
activity in vivo. We isolated two major substances from the LH-20 active
fraction using preparative HPLC. By means of mass spectrometry, 1H-NMR,
and 13C-NMR analyses, one substance was revealed to be
procyanidin B-3 and the other substance was identified as (+)-catechin.
Purified procyanidin B-3 showed high hair-growing activity in the form of in
vitro hair epithelial cell growth-promoting activity and in vivo anagen-inducing
activity; however (+)-catechin showed no hair-growing activity. For the
purpose of examining the hair-growing mechanisms of procyanidin B-3, we
examined its relationship to the TGF-.png)
signal pathway, which is known to be a regulator of catagen induction.
Addition of TGF-1
to hair epithelial cell cultures dose-dependently decreased the cell growth,
and addition of procyanidin B-3 to the culture neutralized the
growth-inhibiting effect of TGF-1.
From these results, it is concluded that procyanidin B-3 can directly
promote hair epithelial cell growth in vitro, has the potential to
counteract the growth-inhibiting effect caused by TGF-1
in vitro, and has potential to stimulate anagen induction in vivo.
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